CALCUSYN MANUAL PDF
年1月23日 CalcuSyn is a software tool whose main purpose is to aid individuals in analyzing mixed drug treatments, a more and more used practice in. combination-index data generated by CalcuSyn software analyses .. and antagonism, respectively (Source: CalcuSyn manual, Biosoft, ). Manual and Software, Biosoft, Cambridge, U.K., Chou, T.-C. and Hayball , M. CalcuSyn for Windows, Multiple-drug dose-effect analyzer and manual.
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Step 7 – Click OK and the drug will be added to the experiment and shown in the contents pane. The combined effects of multiple drugs or enzyme inhibitors.
CalcuSyn was reviewed by Madalina Boboc. There are ten review articles that have been published, including three in encyclopedias 11,13, Select the file and click Open. Data can be processed both for individual drugs and for constant-ratio or non-constant-ratio combinations of drugs.
Welcome to COMPUSYN
The approximated error in CI for the mutually exclusive case now becomes: Double-click on Raw Data to open the raw data grid to enter drug data. As falcusyn as the m and Dm parameters for each single drug are available, the CI values for each data point of the nonconstant ratio design can still be calculated.
Thus, the standard deviation of the dose is estimated as: Unlike empirical or statistical approaches in doseeffect analysis in biology, the mass-action law provides well defined models for mathematical derivations. Derivation and properties of Michaelis-Menten type and Hill type equations for reference ligands.
Using the Program 27 Entering a Combination Drug As with entering a single drug there are two methods to enter a combination drug: Note that until you have entered the correct password, you will not be able to run CalcuSyn at all. Drug combinations are widely used because multiple drugs affect multiple targets and cell subpopulations.
These ambiguous practices generate confusion. Prerequisite Dose-effect Curves Each drug alone should have a dose-effect relationship. A comprehensive manual is supplied as. You can only enter non-constant ratio combinations containing up to 6 drugs using the wizard.
Both potency Dm and manaul m parameters are essential for determining synergism or antagonism. If any CI value s are greater than 2, it is recommended that the antilog of the averaged log CI value is used to avoid significant biases of overestimating antagonism.
The file should be structured as below. Registration The first time you run CalcuSyn, you will be presented with a dialog containing a serial number and cxlcusyn a password.
Analysis of drug interactions.
Instead, potentiation augmentation, enhancement or inhibition suppression can be determined. The simplicity of calchsyn general equations makes for versatility in their applications. Click the Next button to proceed to the next page of the wizard.
The left-hand pane will be populated with the experiment basics. For combinations, in contrast, any number of data points can be used for the CI calculations.
Each calcuayn can have a maximum of 40 pairs of dose and effect data. Using the Program 23 Step 5 – Click the Finish button and the drug will be added to the experiment and shown in the Contents Pane. Note that for two-drug combinations, the CI is the sum of two terms. MTX Fraction affected uM 0. Topotecan Data points entered: Using the Program 41 imports text file data directly into the raw data grid where you can then use calcusny to create drugs and combinations within your CalcuSyn experiment.
You will then be required to enter the dose data for all drugs in your combination. M, mM or IU can be combined and the results analyzed as usual.
Capcusyn is a software tool whose main purpose is to aid individuals in analyzing mixed drug treatments, a more and more used practice in medicine. You will be presented with a standard file save dialog box.
Step 5 – Enter the first datapoint by selecting New. Combination of more than Two Drugs For three drug combinations e.
You can import a minimum of 1 and a maximum of 20 sets of data from a single text file. Data will be imported into the grid and will be placed in the current cell and then outwards right and downwards.
For mutually exclusive drugs, the combination index equation for the combination of n inhibitors 6, 8, 14 can be described by: